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POLCAM is a molecular orientation microscopy method. It allows for the measurement of the orientations of fluorescent molecules; both at the single-molecule (Single-Molecule Orientation Localisation Microscopy, SMOLM) and bulk level (diffraction-limited polarisation microscopy). POLCAM is based on polarised detection using a polarisation camera —a camera that has polarisers integrated onto the sensor —, and is compatible with conventional widefield fluorescence microscopes.

The preprint is available on : https://doi.org/10.1101/2023.02.07.527479

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This website includes a step-by-step guide to allow users to easily modify their systems for use with POLCAM (Hardware) and installation instructions and user manuals for using the accompanying image analysis software.